Protocols
Over the years I have compiled a small list of protocols that should prove very useful for any molecular and cellular biologist. Of course, they are far from the bible, but they do represent a good starting point.
Cloning / Misc.
Preparing Competent DH5alpha cells (and transformation) [html] [word]
Bacterial Expression
GST Protein Purification [html] [word]
His Tag Protein Purification [html] [word]
Western Protocol [html] [word]
Insect Cell Expression
Constructing a Baculovirus (Pharmingen) [html] [word]
Constructing a Baculovirus (Bac-2-Bac) [html] [word]
Infecting Sf9 Cells On Plates [html] [word]
Virus Amplification [html] [word]
Titering Viruses [html] [word]
Mammalian Cell Expression
Cultivation of 293 Cells [html] [word]
Transfection of 293 Cells [html] [word]
Transfection of 293 Cells in 10cm and 15cm Plates [html] [word]
Checking For Expression [html] [word]
Checking For Expression of Fc tagged protein by ELISA [html] [word]
Roller Bottle Cultures [html] [word]
Cryopreservation of 293 Cells [html] [word]
Selenomethionine Labeling of Secreted Proteins [html] [word]