Study Guide III - MoAb's and Peptides

  1. Let's talk MoAbs - download the PPT
    1. How do they differ from peptide/amino acids
      1. Lock and key
      2. Receptor binding
    2. Define the Ab_Ag relationship
      1. Ag - Determinants/epitopes
      2. B lymphocytes - Plasma and Memory cells response
      3. Poly antibody - Polyclonal response
    3. Explain the process on how a MoAb is created from a mouse host and associate correct terminology
      1. Lymphocytes response
      2. Fusing lymphocytes with myeloma cells
      3. Polyethylene glycol
      4. HAT medium
      5. Cloning and separating
      6. Finding the right clone
      7. Propagation
    4. Types of immunoglobulins (IgG)
      1. Whole IgG structure
      2. Fragmented IgG structure
      3. Pepsin
      4. Papain
      5. F(ab')2, Fab, Fc
    5. Fragmented vs whole MoAbs?
      1. Apply the correct radionuclide to the type of MoAb being used
      2. Consider the type of radiation being used: beta vs. gamma
      3. Diagnostic vs. therapeutic
      4. Time between injection and scan
      5. HAMA response
      6. Bi-functional chelator
    6. What is the difference between affinity and avidity
      1. Strength of attraction
      2. Integrity of the bond - consider gamma vs beta
    7. What is the difference between immunoreactive fraction and immunospecificity?
      1. Free MoAb
      2. Loss of specificity of the bond
  2. Compare whole IgG OnocScint to fragmented IgG Arcitumomab. Both are used to diagnose colon cancer.
  3. Related tumor imaging to lab values
    1. Gleason score/sum - link
    2. PSA levels, density, TRUS, and velocity - link
    3. What does it mean when you say the tumor cell is well vs poorly differentiated - link
  4. Prostate Cancer - link
    1. Evaluate non-nuclear procedures used to assess this disease
  5. DOTATATE - link
    1. What is the difference between the different DOTAs
    2. Compare DOTA to Octreotide
    3. 177Lu-DOTA - link
      1. Eight week time lines
      2. What happens during the day of therapeutic administration
      3. Use of antiemetic
      4. Use of amino acids
  6. Pertechnetate physiology -Link
    1. Compare the relationship between interstitial fluid and the vascular pool
    2. Assess how the human system picks up and/or eliminates pertechnetate in the following organs
      1. Kidney
      2. Stomach and GI track
      3. Thyroid
      4. Brain
      5. Choroid Plexus
      6. Sweat glands
    3. Consider the following scenarios
      1. How would Lugol's affect pertechnetate uptake
      2. Questionable results from an In Vivo RBC tag
      3. Not administering KClO4- prior to a pertechnetate brain scan
      4. Looking at a bone scan when is there too much free tech?

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11/23