05. Gupta, Ram B.; Han, Chae J.; Johnston, Keith P. Recovery of proteins and amino acids from reverse micelles by dehydration with molecular sieves. Biotechnol. Bioeng. (1994), 44(7), 830-6.

Abstract

A new method is presented to ppt. proteins and amino acids from reverse micelles by dehydrating the micelles with mol. sieves. Nearly complete pptn. is demonstrated for a-chymotrypsin, cytochrome c, and tryptophan from 2-ethylhexyl sodium sulfosuccinate (AOT)/isoctane/water reverse micelle solns. The products ppt. as a solid powder, which is relatively free of surfactant. The method does not require any manipulation of pH, ionic strength, temp., pressure, or solvent compn., and is applicable over a broad range of these properties. This general approach is compared with other techniques for the recovery of biomols. from reverse micelles.

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