Reduced Particles MAA Worksheet

Name ______________________________

  1. Dilute MAA vial with 5 mL of saline. Withdraw a 1 mL aliquot and place it in a 30 mL sterile empty vial.
    Calculate the total # of particles in the 1 mL aliquot:

    2. Total particles in vial = ______________________

    Step 1

  2. Calculate total volume needed to make the kit of reduced particles (all contents go into a 30 mL sterile vial)

    3. Step 2

  3. Calculate the activity of 99mTc required for patient dose. See dose ranges.
    4. Dose in mCi x total volume (activity x volume (step 2) define total 99mTc in mCi.
    Adult dose is usually 3.5 mCi, however, pediatric dose must be acquired by the physician.
    Total activity is then determined below:

    Step 3

  4. From the elution vial add the required 99mTc activity to the 30 mL vial and note its volume.

    5. Step 4

  5. Incubate for 15 minutes. Smaller particle amount may require up to 30 minutes incubation.

  6. Calculate the volume of normal saline needed in the 30 mL vial.

    7. Step 5

  7. Complete TLC on the final product.

Return to Table of Content

For your homework Calculate the reduced particles on a pediatric patient given the following data: pediatric dose = 1.5 mCi should contain 75,000 particles. The MAA vial contains 4.5 million particles and the elution vial of pertechnetate contains 85 mCi/mL. Once you have completed your calculations, you can evaluate your answer by clicking here.