Take home Exam III - MoAb's and Peptides

1. Let's talk MoAbs - download the PPT (answer all questions in #1)
   1. Compare the differ between peptide/amino acids
      1. Lock and key
      2. Receptor binding
   2. Define the Ab_Ag relationship
      1. Ag - Determinants/epitopes
      2. B lymphocytes - Plasma and Memory cells response
      3. Poly antibody - Polyclonal response to disease
   3. Explain the process on how a MoAb is created from a mouse host with associate correct terminology
      1. Lymphocytes response
      2. Fusing lymphocytes with myeloma cells
      3. Polyethylene glycol
      4. HAT medium
      5. Cloning and separating
      6. Finding the right clone
      7. Propagation
   4. Identify the parts of an immunoglobulins (IgG)
      1. Whole IgG structure
      2. Fragmented IgG structure
      3. Pepsin the fragmented becomes ____
      4. Papain the fragmented becomes ____
      5. F(ab')₂, Fab, Fc
   5. Fragmented vs whole MoAbs?
      1. Apply the correct radionuclide to the type of MoAb being used (Frag vs Whole)
      2. Consider the type of radiation being used and tag an beta vs. gamma emitter
      3. Diagnostic vs. therapeutic - Make a suggestion for each and explain your reasoning
      4. Time between injection and scan (Frag vs Whole)
      5. Based on the type of IgG what might the concern be when it comes to the HAMA response
      6. Bi-functional chelator - give an example of where this would be applied
   6. What is the difference between affinity and avidity?
      1. Strength of attraction
      2. Integrity of the bond - consider gamma vs beta
   7. What is the difference between Immunoreactive fraction and Immunospecificity?
      1. Free MoAb
      2. Loss of specificity of the bond
2. Compare whole IgG OnocScint to fragmented IgG Arcitumomab. Both are used to diagnose colon cancer (choose between #2 and #3)
   1. Target to Bkg
   2. Time between injection and scan
   3. Radioisotope being utilized
3. Related tumor imaging to lab values
   1. Explain the difference between Gleason score and sum - link
   2. How are the following values applied to assess prostate cancer involvement - PSA levels, density, TRUS, and velocity - link
   3. What does it mean when you say the tumor cell is well vs poorly differentiated - link
4. Prostate Cancer - link (choose between #4 and #5)
   1. Evaluate non-nuclear procedures used to assess this disease. What appears to be the gold standard? Or is there one?
   2. Understand the different therapeutic approaches to treat this disease (give two non-NMT examples)
   3. Consider the ProstaScint imaging procedure and know these details
      1. When should you scan - note there can between timelines
      2. Why is a vascular imaging required either thirty minutes post dose of ProstaScint and 72 hours RBC scan
      3. Is HAMA a concerns?
   4. Compare FDG, Fluciclovine, and PSMA for the application in prostate imaging
      1. Biodistribution
      2. Initial staging vs restaging
5. DOTATATE - link
1. What is the difference between the different DOTAs?
2. Compare DOTA to Octreotide
3. ¹⁷⁷Lu-DOTA - link
   1. What happens during the day of therapeutic administration
   2. Identify the method used administer this therapeutic
   3. What is the timeline and the amount of therapeutic doses during the total duration of this treatment
   4. What is the purpose of using a antiemetic?
   5. What is the purpose of using amino acids?

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