Biol 213: Genetics (Fall 2001)
Genetics Lab
Lab Syllabus

Lab 1: Serial dilution of a bacterial culture
Problem: Figure out volumes used in mixing two pure cultures together
     Notes on reports                Sample lab report

Lab 2: Isolation DNA fragment from plasmid DNA for cloning
Problem: Identify plasmid and figure out how to clone a given fragment from it
     Gels from Lab 2: Section 2
     Important Clue: Supercoiling of DNA within living cells
     Notes on reports                Sample lab report

Lab 3: Genetic analysis of mystery mutant of Drosophila melanogaster
Problem: Characterize three mutant strains and map the underlying mutations
     PC-Fly: Instruction Manual
     How to refine map distance calculations
     Notes on Progress Reports

Lab 4: Recombination and complementation to map genes of bacteriophage T4
Problem: Map two mutations within rII region of T4

Lab 5: Regulation of the lac operon
Problem: Identify lac mutations in four mystery strains

Lab 6: The Agrobacterium tumefaciens Genome Project
Problem: Figure out all you can about a newly sequenced region from the Agrobacterium genome
     Initial instructions on analyzing mystery sequence
     2nd Annual UR Symposium on Agrobacterium Genomics: Program