BNFO 300 
Molecular Biology Through Discovery
Path to class (Tuesday, Sep 10) - Hemoglobin and Perutz et al (1965)
(please press SUBMIT button when finished)
Fall 2019  


My advice on the matters for the day (as listed in the calendar):

Protein
  • Hemoglobin Tutorial: Main topic for today. Section III of the companion should be of help (make sure the version in the upper right corner is September 6 or later). Parts 2, 3, 4 and beginninf of 5 are the most important parts of the tutorial. Shouldn't be a big time expenditure.
     
  • Perutz et al (1965): Just make sure you've downloaded the right article, then skim Sections I and II of the companion to the article. Section III is more important, but that's related to the Hemoglobin Tutorial, described above.
     
  • Problem Set 2, Problem 3: Make some progress with the community approach described in Problem Set 2, problem 4, to reproducing Sanger and Tuppy's work. We'll discuss progress and continue in class.
     
  • Problem Set 2, Problem 2: This class is your best chance to get feedback on this problem before the target date. It might be a good to take a look at it.
Research Project
  • How to create a bibliography and List of methods: Don't worry about these. I'll provide an overview in class.


I. Basic Information

A. Your name 
II. Old business (no need to repeat anything you've said in a previous questionnaire)
  1. Sanger & Tuppy (1953)
    Anything further you'd like to discuss regarding the article Sanger & Tuppy (1953) and its companion?
    Do you understand how to go from observations (raw chromatographic results) to sequences of peptides?
    To long sequences from overlapping peptides?
    Were you able to answer Study Question 17 (creating two chromatograms that led to the conclusions listed in Sager & Tuppy's Table 3 Spot 1)?

     
  2. Problem Set 2, Problem 3
    Progress in the community project? Any issues?
III. Perutz et al (1965)
A. Have you found and downloaded the article Perutz et al (1965) cited in the companion?
    Are you sure you got the right one? How do you know?
   

B. How far have you gotten in a productive reading of the article?
    You can answer by referring to the last section you read or the last study question you addressed (e.g. SQ12) in the companion.
   

Consider how comfortable you are with the following topics (as defined by the companion to Perutz et al):

  • The overall questions addressed by the article
  • The connection between Fig. 1 and Table 2
  • Study questions 1 and 2

With this in mind, how could class time best be spent to meet your needs?
As always, refer to specific matters and relate your current understanding and efforts you've made to address your discomfort.
Do you have any other questions concerning the material in the notes?

IV. Hemoglobin Tutorial
A. Were you able to access Hemoglobin Structure?
   

Consider how comfortable you are with the following topics (as defined by the Hemoglobin tutorial and the companion to Perutz et al):

  • The different ways protein structures are presented graphically, pros and cons
  • The overall structure of hemoglobin
  • The basis for the formation of alpha helices
  • The role of hydrophobicity in the formation of hemoglobin

With this in mind, how could class time best be spent to meet your needs?
As always, refer to specific matters and relate your current understanding and efforts you've made to address your discomfort.
Do you have any other questions concerning the material in the notes?

V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?

Thanks!

REMEMBER TO CLICK SUBMIT!