BNFO 300 
Molecular Biology Through Discovery
Questionnaire on notes: DNA Replication
(please press SUBMIT button when finished)
Spring 2017  


I. Basic Information

A. Your name  
II. Old business (no need to repeat anything you've said in a previous questionnaire)
  1. Problem Set 3
         Anything further you'd like to discuss regarding Problem Set 3?
        
     
  2. DNA Structure
         Anything further you'd like to discuss regarding the notes DNA Structure and the notes on diffraction?
         How the helical structure of DNA and internucleotide distances can be discerned from Franklin and Gosling's x-ray photograph?
         The nature and biological roles for palindromic sequences?
        
     
  3. How to read a research article
         Anything further you'd like to discuss regarding the mini-article (describing an experiment concerning DNA replication) and the suggestions how to go about reading it (and any research article)?
        
III. DNA replication

A. How far have you gotten in the part 1 of the notes entitled DNA Replication, Part I?
     

B. Consider how comfortable you are with the following topics regarding DNA replication:

  • The need for a pre-existing 3'-OH end, generally an RNA primer
  • The concepts of leading and lagging strands
  • The requirement that DNA synthesis always proceeds 5' to 3'
  • Proofreading and accuracy
     
IV. Meselson and Stahl

A. How far have you gotten in Meselson and Stahl (1958) and its companion?
     

B. Consider how comfortable you are with the following topics regarding Meselson & Stahl:

  • The topological problem implicit in the replication model presented by Watson and Crick
  • Dispersive replication, and how it addresses the topological problem
  • The critical differences between the three featured models of DNA replication
  • How heavy isotopes can distinguish new from old DNA
  • What properties of cesium chloride are helpful in this procedure
  • Equilibrium density centrifugation
  • How to read Fig. 4 (e.g. what do position and ultraviolet absorption indicate)
  • The relationship between Meselson and Stahl's results and the predictions of the three featured replication models
  • How Delbrück's topological problem was ultimately resolved

      Please relate your thoughts
     

V. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?

Thanks!

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