BNFO 300 
Molecular Biology Through Discovery
Questionnaire on: DNA Replication and Meselson & Stahl (1958)
(please press SUBMIT button when finished)
Spring 2016  


I. Basic Information

A. Your name  
II. Old business (no need to repeat anything you've said in a previous questionnaire)

A. How to read a research article
     Anything further you'd like to discuss regarding the mini-article (describing an experiment concerning DNA replication) and the suggestions how to go about reading it (and any research article)?
    

B. DNA Replication
     Anything further you'd like to discuss regarding the notes DNA Replication, Part I?
     Why a need for a free 3'-OH end and an RNA primer? Why 5' to 3'?
    

III. Meselson and Stahl

A. How far have you gotten in Meselson and Stahl (1958) and its companion?
     

Consider how comfortable you are with the following topics regarding Meselson & Stahl:

B. The topological problem implicit in the replication model presented by Watson and Crick

C. Dispersive replication, and how it addresses the topological problem

D. The critical differences between the three featured models of DNA replication

E. How heavy isotopes can distinguish new from old DNA

F. What properties of cesium chloride are helpful in this procedure

G. Equilibrium density centrifugation

H. How to read Fig. 4 (e.g. what do position and ultraviolet absorption indicate)

I. The relationship between Meselson and Stahl's results and the predictions of the three featured replication models

J. How Delbrück's topological problem was ultimately resolved

      Please relate your thoughts (not just letters), but no need to repeat anything you've previously submitted
     

IV. Miscellaneous
Any other comments, questions, suggestions, or concerns you may have?

Thanks!

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