BNFO 300 
Molecular Biology Through Discovery
Questionnaire on: Belasco et al (1985) - Regulation of transcription
(please press SUBMIT button when finished)
Fall 2014  


I. Basic Information

A. Your name 
II. Old business
(no need to repeat anything you've said in a previous questionnaire)
  1. Research proposal?
    Progress? Problems? Progress on summary?
    Have you looked ahead to the proposal outline (target date Nov 14)? Approaching readiness?
  2. Brenner et al (1961)?
    Final words on Brenner et al (1961) and its companion?
    Do you grasp the general outline of the Pardee, Jacob, and Monod experiment?
    Do you grasp the general outline of the Riley and Pardee experiment?
    Do you understand the differences amongst the three models presented by Brenner et al?
    Do you understand the principle behind their experiment?
    Do you understand how to interpret Figs. 2 - 8?

III. Belasco et al (1985), Part I

A. How far have you gotten in this article and its companion (Part I)?
     

B. Consider how comfortable you are with the following topics regarding these matters:

  • The question you are directing at this article and the biology behind the question
     
  • The nature of an operon
     
  • The process of skimming an article to decide what part of it to read
     
  • S1 protection experiments
     

C. Choose up to five study questions that you would most like discussed in class and explain why.
     

IV. Belasco et al (1985), Part II

A. How far have you gotten in this article and its companion (Part II)?
     

B. Consider how comfortable you are with the following topics regarding these matters:

  • The elements of the S1-protection experiment in this article
     
  • Use of BioBIKE to investigate genome of R. capsulatus
     
  • Equating Belasco et al's Fig. 1 with the genome sequence obtained from BioBIKE
     
  • Identification of restriction sites (e.g. EcoRI)
     
  • Interpreting Fig. 2 from Belasco et al
     
  • Interpreting Fig. 4 from Belasco et al
     

C. Choose up to five study questions that you would most like discussed in class and explain why.
     

V. Miscellaneous

Any other comments, questions, suggestions, or concerns you may have?

Thanks!

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