BNFO 300 
Molecular Biology Through Discovery
Questionnaire on: DNA Replication and Meselson & Stahl (1958)
(please press SUBMIT button when finished)
Fall 2013 


I. Basic Information

A. Your name 
II. Old Business

(No need to respond to any section if you've done so in an earlier questionnaire and nothing's changed)

A. Benzer (1959)
Any final concerns about Benzer (1959) and related matters?
     

B. Poorly defined problems, like Problem Set 4, #5
We spent some time on such problems this past Tuesday. Do you have an idea how to go about making progress with them?
     

C. Research Proposal
Have you met with your mentor? Do you have an idea how to proceed (e.g. with the bibliography)?
     

D. Anything else from previous material?
     

III. DNA replication, Part 1

A. How far have you gotten in the part 1 of the notes?
     

Consider how comfortable you are with the following topics regarding DNA replication:

B. The need for a pre-existing 3'-OH end, generally an RNA primer

C. The concepts of leading and lagging strands

D. The requirement that DNA synthesis always proceeds 5' to 3'

E. Proofreading and accuracy
     

Choose up to two study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6 

IV. Meselson and Stahl

A. How far have you gotten in the companion to Meselson and Stahl?
     

Consider how comfortable you are with the following topics regarding Meselson & Stahl:

B. The topological problem implicit in the replication model presented by Watson and Crick

C. Dispersive replication, and how it addresses the topological problem

D. The critical differences between the three featured models of DNA replication

E. How heavy isotopes can distinguish new from old DNA

F. Equilibrium density centrifugation

G. How to read Fig. 4

H. The relationship between Meselson and Stahl's results and the predictions of the three featured replication models

I. How Delbrück's topological problem was ultimately resolved
     

Choose up to six study questions that you would most like discussed in class:

SQ1  SQ2 SQ3 SQ4 SQ5 SQ6  SQ7  SQ8  SQ9 
SQ10 SQ11 SQ12 SQ13  SQ14 SQ15 SQ16 SQ17 
SQ18  SQ19 
IV. Miscellaneous

Any other comments, questions, suggestions, or concerns you may have?

Thanks!

REMEMBER TO CLICK SUBMIT!