13 Jan *LAB 1. Calibration and dilution
      (Brief report due 20 Jan, 10 points)

20 Jan *LAB 2. Spontaneous vs directed mutation
      Based on Luria & Delbrück (1943) paper
      (Full report due 3 Feb, 30 points)

27 Jan LAB 3: Regulation of the lac operon
      (Brief report due 10 Feb, 15 points)

3 Feb *LAB 4: Nature of the regulation of lac
      Based on Pardee et al (1959) paper
      (Full report due 17 Feb, 30 points)

10 Feb LAB 5: Conjugation - Gradient of transmission
      (Brief report due 24 Feb, 15 points)

LAB 6: Three point cross, Part A: Obtain P1 lysogen
      (Full report due 17 Mar, 30 points)

17 Feb LAB 5 Conjugation (cont) and LAB 6 (cont): Three point cross

24 Feb LAB 6 (cont): Three point cross\

3 Mar LAB 7: Characterization of transposon insertion
      Part A: Screen of Tn5lac mutagenesis
      (Full report due by 1 May, 60 points)

10 Mar NO LAB -- MIDTERM BREAK

17 Mar LAB 7, Part B: Isolation of chromosomal DNA from mutant

24 Mar LAB 7, Part C: Cloning of region around Tn5lac insertion

30 Mar to end of semester: *LAB 7: (continue at your own schedule)
      Restriction digestion of cloned region
      Mapping of insertion by conjugation and transduction
      Mapping by Southern blot
      Characterization of regulation of mutated gene

1 May LAB 7 (LAST DAY ACCEPTED)

*You are responsible for devising experimental protocols for all labs marked with an asterisk.